Estimation and validation of entecavir in bulk and pharmaceutical dosage forms by UV spectrophotometry

Abstract

A simple, sensitive and economical UV-Spectrophotometric method was developed for the estimation of Entecavir in bulk and pharmaceutical dosage form. The absorption spectra in the proposed method, λ_max of Entecavir was found to be 254nm. The calibration curve was linear over the concentration range of 3-18μg/ml with correlation coefficient of 0.999. A Percentage recovery of Entecavir ranges from 99.53-100.41%w/w indicating that the developed method is accurate and % RSD was less than 2 which indicates a good accuracy of the method. The limit of detection and limit of quantization were found to be 1.25μg/ml &3.81μg/ml. The proposed method will be suitable for the analysis of Entecavir in bulk and pharmaceutical formulation.