Genotyping analysis of tumor necrosis factor –alpha and lysyl oxidase- like 1 genes in patients with pseudoexfoliation syndrome

Abstract

Pseudoexfoliation syndrome (PEX) is an age-related systemic disorder characterized by deposition of whitish-gray pseudoexfoliation fibrillogranular amyloid like material in several intraocular and extraocular tissues. The present study was carried out to investigate the association of Tumor Necrosis Factor-alpha and lysyl oxidase like 1 (LOXL1) genes variants with pseudoexfoliation syndrome in the Iraqi population.  The coding regions of TNF-α and LOXL1genes were fully sequenced in 45 clinically diagnosed PEX patients and 30 healthy controls. The regions of TNF-α and LOXL1 genes with their single nucleotide polymorphisms (SNPs) were amplified and sequenced. The single nucleotide polymorphism of Tumor necrosis factor-alpha G 308 A was not statistically significant (P=0.545), whereas, GG genotype and allele G of lysyl oxidase-like 1 were statistically significant among PEX patients, (P=0.0003 and P <0.0001, respectively). GG genotype G allele polymorphism of lysyl oxidase like 1 is mainly expressed among PEX patients and susceptibility with disease might be prospected.