The influence of an organic selenium (IV) compound on progression of tumour induced using prostate cancer cells and gene expression connected to the oxidative stress response

Abstract

Analyse the influence of Selol on progression of tumour and the changes in gene expression, connected to the cellular oxidative stress response, in cells taken from tumours induced in immunodeficient mice through subcutaneous inoculation with malignant LNCaP prostate cells. Based on the PSA levels (at 5 weeks following inoculation of LNCaP), the mice were divided into three groups. Selol and a placebo were given for the following 3 weeks. After this time, the animals were sacrificed, blood was taken (PSA) and tumours were isolated. RNA from samples was analysed using Real-Time PCR. Treating mice with Selol (21 days at a dose of 17 mg/kg of b.m.) resulted in a drop in the rate of body mass reduction and stopped the increase of plasma PSA levels. The fold changes in expression of the genes, in cells derived from tumours, treated with Selol, in the array, apart from 1 (NME5), failed to exceed the required 2-fold change. Treating tumour-bearing mice with Selol resulted in a reduced rate of body mass loss and halted the increase in plasma PSA concentration, then compared to mice receiving the placebo. The expression of genes, involved in oxidative stress following treatment with Selol, in LNCaP cells, didn’t change.