The influence of an organic selenium (IV) compound on progression of tumour induced using prostate cancer cells and gene expression connected to the oxidative stress response

Authors

  • Iza Ksiazek Department of Biochemistry and Biofarmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw
  • Karolina Sitarz Department of Biochemistry and Biofarmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw
  • Magdalena Roslon Department of Biochemistry and Biofarmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw
  • Elzbieta Anuszewska Department of Biochemistry and Biofarmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw
  • Grazyna Hoser Department of Clinical Cytology, The Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw
  • Jadwiga Dudkiewicz Wilczyńska Department of Biochemistry and Biofarmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw
  • Marzena Iwanowska Department of Laboratory Diagnostics, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw
  • Piotr Suchocki Department of Bioanalysis and Drug Analysis, Medical University of Warsaw, Banacha 1, 02-097 Warsaw and Department of Pharmaceutical Chemistry, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw, Poland

Keywords:

Selol, LNCaP, Real-Time PCR

Abstract

Analyse the influence of Selol on progression of tumour and the changes in gene expression, connected to the cellular oxidative stress response, in cells taken from tumours induced in immunodeficient mice through subcutaneous inoculation with malignant LNCaP prostate cells. Based on the PSA levels (at 5 weeks following inoculation of LNCaP), the mice were divided into three groups. Selol and a placebo were given for the following 3 weeks. After this time, the animals were sacrificed, blood was taken (PSA) and tumours were isolated. RNA from samples was analysed using Real-Time PCR. Treating mice with Selol (21 days at a dose of 17 mg/kg of b.m.) resulted in a drop in the rate of body mass reduction and stopped the increase of plasma PSA levels. The fold changes in expression of the genes, in cells derived from tumours, treated with Selol, in the array, apart from 1 (NME5), failed to exceed the required 2-fold change. Treating tumour-bearing mice with Selol resulted in a reduced rate of body mass loss and halted the increase in plasma PSA concentration, then compared to mice receiving the placebo. The expression of genes, involved in oxidative stress following treatment with Selol, in LNCaP cells, didn’t change.

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Published

2014-10-01

How to Cite

Iza Ksiazek, Karolina Sitarz, Magdalena Roslon, Elzbieta Anuszewska, Grazyna Hoser, Jadwiga Dudkiewicz Wilczyńska, Marzena Iwanowska, & Piotr Suchocki. (2014). The influence of an organic selenium (IV) compound on progression of tumour induced using prostate cancer cells and gene expression connected to the oxidative stress response. World Journal of Pharmaceutical Sciences, 2(10), 1146–1158. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/influence-selenium-compound-tumour-oxidative-stress

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Section

Research Article