Preparation and evaluation of ampicillin solid lipid nanoparticles

Abstract

Solid lipid nanoparticles (SLNs) have been studied as a drug-delivery system for the controlling of drug release. These systems have many important advantages, such as biocompatibility, good tolerability, and ease of scale-up. Ampicillin as a β-lactam antibiotic was studied to load on SLNs for control of drug release to increase administration intervals and decrease dose of drug to increase patient compliance and decrease antibiotic resistance. The size of ampicillin loaded nanoparticles, drug loading, drug release profile, morphology and antibacterial effect were studied. The conventional broth macrodilution tube method was used to determine the minimum inhibitory concentration (MIC) and minimum bacteriostatic concentration (MBC) of ampicillin SLNs with respect to P.aeruginosa, E. coli and S. aureus in vitro. Prepared particles show 150 nm of size. Drug loading efficiency was 77±3%, all prepared particles had spherical shape. After 24 hours more than 95% of loaded drug was detected in release samples. MIC and MBC of ampicillin loaded nanoparticles decreased in comparison with free ampicillin against P.aeruginosa, E. coli and S. aureus.