Comparative qualitative analysis of callus extracts of in-vitro and in-vivo plants of Jasminum angustifolium, a wild and medicinal plant

Abstract

Plant tissue plays an important role in the production and conservation of medicinal plants. The present study reveals that callus was raised from the leaf explants of Jasminum angustifolium. The leaf and stem explants of Jasminum angustifolium were used for callus regeneration on MS medium supplemented with 3% sucrose with different concentrations of 2, 4-D and BAP. A maximum callus was obtained from leaf explants when cultured on MS medium supplemented with 2, 4-D and BAP at 2.0mg/litre respectively. The callus obtained in 2, 4-D concentration was best and shows 73% of callus growth than BAP. The callus used in this study was taken after eight to nine weeks of growth. The qualitative analysis of this study reveals that the secondary metabolites have the ability to produce the changes in the human body. The qualitative analysis shows the presence of tannins, saponins, terpenoids and quinones was observed in leaf callus. The wild leaf extracts shows the presence of tannins, saponins, terpenoids, and quinones.