Quantitative simultaneous determination of montlukast and bambuterol in combined tablet formulation by RP-HPLC method

https://doi.org/10.54037/WJPS.2022.100904

Authors

  • C. P. Vaishnavi M. Pharmacy, Department of Pharmaceutical Analysis, Centre for Pharmaceutical Sciences, Jawaharlal Nehru Technological University, KPHB Colony, Kukatpally, Hyderabad - 500085, Telangana, India.
  • Makula Ajitha Professor, Center for Pharmaceutical Sciences, JNTU, Hyderabad.

Keywords:

Bambuterol, Montelukast, RP-HPLC, Method Development, Validation

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Bambuterol and Montelukast in bulk and Tablet dosage form. Chromatogram was run through Std Discovery 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 254.0 nm. Retention time of Bambuterol and Montelukast were found to be 2.067min and 3.032 min. %RSD of the Bambuterol and Montelukast were and found to be 0.6% and 0.4% respectively. %Recovery was obtained as 100.12% and 99.96% for Bambuterol and Montelukast respectively. LOD, LOQ values obtained from regression equations of Bambuterol and Montelukast were 0.09, 0.26 and 0.14, 0.42respectively. %Assay was obtained as 99.97% and 100.02% for Bambuterol and Montelukast respectively. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Downloads

Published

2022-09-05

How to Cite

C. P. Vaishnavi, & Makula Ajitha. (2022). Quantitative simultaneous determination of montlukast and bambuterol in combined tablet formulation by RP-HPLC method: https://doi.org/10.54037/WJPS.2022.100904. World Journal of Pharmaceutical Sciences, 10(09), 42–51. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/bambuterol-montelukast-hplc-method

Issue

Section

Research Article

Similar Articles

You may also start an advanced similarity search for this article.