UV spectroscopic estimation of oseltamivir in bulk drug and formulation

Abstract

A simple, accurate and precise UV spectroscopy method has been developed for the estimation of Oseltamivir. It is a medication used for the treatment of influenzas A and influenza B. It inhibits the influenza's neuraminidase enzyme. The enzyme cleaves the sialic acid which is found on glycoproteins on the surface of human cells that helps new virions to exit the cell. Here we have developed a UV spectroscopic method for the quantitative determination of Oseltamivir in the bulk and tablet dosage forms. The parameters linearity, precision, accuracy, limit of detection and limit of quantification were studied according to international conference on harmonization guidelines (ICH). The determination was carried out at an absorption maximum of 218 nm using distilled water as solvent. In the present method, linearity over the concentration range of Oseltamivir was found to be 10-50 µg/ml, with a correlation coefficient of 0.9998. The results of analysis have been validated statistically for linearity, accuracy and precision, LOD and LOQ of the processed method. The developed method was successfully applied for the quantitative analysis of commercially available dosage form.