RP HPLC METHOD FOR DETERMINATION OF DASIGLUCAGON IN PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, precise, accurate sensitive and specific RP-HPLC method for the determination of Dasiglucagon in pharmaceutical dosage form. Chromatogram was run through Kromosil 150 Acetonitrile: Orthophosphoric acid taken in the ratio 70:30 was pumped through column at a flow rate of 1.0ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 270.0nm. Retention time of Dasiglucagon was found to 2.096min. %RSD of the Dasiglucagon were and found to be 0.3%. %RSD of Method precision of Dasiglucagon was found to be 0.6%. %Recovery was obtained as 99.59% for Dasiglucagon. LOD, LOQ values obtained from regression equation of Dasiglucagon were 0.04, 0.11, Regression equation of Dasiglucagon is y = 135481x + 6696.7 Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.