ESTIMATION OF VILOXAZINE BY USING RP- HPLC METHOD

Abstract

The RP-HPLC technology was used to produce a straightforward, precise, and easy-to-understand approach for the quantification of Viloxazine. The conditions used in chromatography are the stationary phase The Azilent measuring 150 mm x 4.6 mm x 5 meters The following parameters were maintained: mobile phase diluent: 0.01N KH2po4: acetonitrile in a 70:30 ratio; flow rate: 1.0 ml/min; detection wavelength: 222 nm; column temperature: 30oC; and so on. Conditions were fine-tuned to provide the best possible outcome. The findings of studying the system suitability characteristics by injecting the standard six times were much lower than the acceptability criterion. After conducting a linearity analysis from 25% to 150%, the R2 value was determined to be 0.999. The results showed that the repeatability was 0.6 and the intermediate precision was 0.4.2. The limits of detection (LOD) are 0.07μg/ml and the limits of quantification (LOQ) are 0.20μg/ml. With the aforementioned procedure, 99.86% of the advertised formulation was detected. In all cases, the purity threshold was more than the purity angle and within the permissible range, according to the Viloxazine degradation experiments. We did not conduct a full-length technique analysis; nonetheless, this approach has the potential to be used for regular Viloxazine analysis