ESTIMATION OF TRILACICLIB BY USING RP-HPLC METHOD

Abstract

A simple, precise, and accurate RP-HPLC method was devised to estimate Trilaciclib. Stationary phase Agilent C18 (150mm*4.6mm3.6m), mobile phase 0.01N KH2PO4: Methanol in the ratio 55:45, flow rate 0.9ml/min, detection wave length 253nm, column temperature 30oC, and diluent mobile phase. Optimized conditions were set. System appropriateness characteristics were examined by injecting the standard six times and scoring considerably below acceptability. R2 was 0.999 for linearity study between 25% and 150%. Precision was 0.9 for repeatability and 0.7 for intermediate. The LOD and LOQ are 0.14μg/ml and 0.41μg/ml, respectively. The aforesaid approach assayed commercial formulation and found 100.31%. All Trilaciclib degradation investigations showed purity thresholds greater than purity angle and within acceptable limits. Full length approach was not conducted; if done, it may be utilized for ordinary Trilaciclib analysis.