DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND TENOFOVIR IN BULK AND TABLET DOSAGE FORM

Abstract

A simple, accurate, exact approach was devised to estimate Lamivudine and Tenofovir in tablet form. Chromatogram was analyzed using Kromosil C18 150 mm (4.6 x 150mm, 5μm). Mobile phase: Buffer (Disodium phosphate) 75% Acetonitrile: 25% injected through column at 1 ml/min. The temperature was 30°C. Optimal wavelength was 250.0 nm. Lamivudine and Tenofovir had 2.250 and 2.875min retention times. %RSD of Lamivudine and Tenofovir were 0.4 and 0.2.% Lamivudine recovered 99.92% and Tenofovir 99.62%. Lamivudine and Tenofovir regression models yielded LOD, LOQ values of 0.11, 0.32, and 0.27, 0.83. Lamivudine regression equation is y = 8638.7x + 10272, Tenofovir 12913x + 5402. The technique devised was straightforward and affordable for routine quality control tests in industries since retention and run times were reduced.