METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF OLANZAPINE AND SAMIDORPHAN BY USING RP –HPLC METHOD

Abstract

The estimate of Samidorphan and Olanzapine in tablet form is simple, accurate, and precise. Chromatograms were examined using ZORBAX Eclipse Plus C18, 4.6mm x 100mm, 5 μm. A 0.01N Ammonium Formate: Acetonitrile 80:20 mobile phase was pumped through the column at 1 ml/min. Temperature was 30°C. The optimal wavelength was 268 nm. Retention time of Samidorphan and Olanzapine was 2.232min and 2.706, respectively. %RSD was 0.4 and 0.2. Samidorphan recovered 99.80% and Olanzapine 99.77%. LOD, LOQ values from regression equations of Samidorphan and Olanzapine were 0.06, 0.17, 0.09, 0.28. Samidorphan and Olanzapine regression equations are y = 31939x + 4951.1. The method was simple and economical, reducing retention and run times for regular quality control tests in industries. Regression equations for Samidorphan and Olanzapine are 31939x + 4951.1 and 37650x + 4959.1, respectively. Retention times and run time were reduced, making the method simple and cost-effective for industrial quality control tests.