STABILITY INDICATING DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND DOLUTEGRAVIR RP-HPLC METHOD
Keywords:
Lamivudine and Dolutegravir, RP – HPLCAbstract
Lamivudine and Dolutegravir were developed with Std Discovery 250 x 4.6 mm, 5m. Buffer-containing MP OPA :MeCN in the 55:45 ratio was poured across the column at 1 ml/min. This procedure employed 0.1% Perchloric acid buffer. The temperature was 30°C. Optimised wavelength was 230 nm. Lamivudine and Dolutegravir had 2.146 and 2.770 min retention times. Lamivudine and Dolutegravir had 0.4 and 0.5 RSD. %Recovery was 100.09% for Lamivudine and 100.62% for Dolutegravir. Lamivudine and Dolutegravir regression equations yielded LOD, LOQ values of 0.24, 0.73, and 0.15, 0.45. The regression equations for Lamivudine and Dolutegravir are y = 91520.x + 1773.9 and y = 179637x + 22360, respectively. Reduced retention and run time for better method development.
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