STABILITY INDICATING DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR RP-HPLC METHOD

Abstract

Sofosbuvir and Velpatasvir were developed with Std Discovery 250 x 4.6 mm, 5m. Buffer-containing MP OPA :MeCN in the 55:45 ratio was poured across the column at 1 ml/min. This procedure employed 0.1% Perchloric acid buffer. The temperature was 30°C. Optimised wavelength was 230 nm. Sofosbuvir and Velpatasvir had 2.146 and 2.770 min retention times. Sofosbuvir and Velpatasvir had 0.4 and 0.5 RSD. %Recovery was 100.09% for Sofosbuvir and 100.62% for Velpatasvir. Sofosbuvir and Velpatasvir regression equations yielded LOD, LOQ values of 0.24, 0.73, and 0.15, 0.45. The regression equations for Sofosbuvir and Velpatasvir are y = 91520.x + 1773.9 and y = 179637x + 22360, respectively. Reduced retention and run time for better method development.