STABILITY INDICATING DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR RP-HPLC METHOD

Authors

  • Vineela Yarasingi M. Pharmacy, M. Pharmacy, Department of Pharmaceutical Analysis, Malla Reddy Institute of Pharmaceutical science, Maisammaguda, Secunderabad, Hyderabad, 500100, Medchal District

Keywords:

Sofosbuvir and Velpatasvir, RP – HPLC

Abstract

Sofosbuvir and Velpatasvir were developed with Std Discovery 250 x 4.6 mm, 5m. Buffer-containing MP OPA :MeCN in the 55:45 ratio was poured across the column at 1 ml/min. This procedure employed 0.1% Perchloric acid buffer. The temperature was 30°C. Optimised wavelength was 230 nm. Sofosbuvir and Velpatasvir had 2.146 and 2.770 min retention times. Sofosbuvir and Velpatasvir had 0.4 and 0.5 RSD. %Recovery was 100.09% for Sofosbuvir and 100.62% for Velpatasvir. Sofosbuvir and Velpatasvir regression equations yielded LOD, LOQ values of 0.24, 0.73, and 0.15, 0.45. The regression equations for Sofosbuvir and Velpatasvir are y = 91520.x + 1773.9 and y = 179637x + 22360, respectively. Reduced retention and run time for better method development.

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Published

2024-09-26

How to Cite

Vineela Yarasingi. (2024). STABILITY INDICATING DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR RP-HPLC METHOD. World Journal of Pharmaceutical Sciences, 12(03). Retrieved from https://wjpsonline.com/index.php/wjps/article/view/1647

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Section

Research Article