METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF MEMANTINE AND DONEPEZIL IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Abstract

For the reason of evaluating memantine and donepezil in tablet dose form simultaneously, a simple, accurate, and precise technique was established. An Agilent 150mm 4.6mm 5μ filter was used to perform a chromatogram. A mobile phase comprising phosphate buffer and acetonitrile in a ratio of 58:42 with flow rate of 1.0 ml/min. A constant 30°C was maintained. The ideal wavelength for memantine and donepezil was 294 nm. Memantine's and donepezil's retention times were discovered to be 2.190 and 2.968 minutes, respectively. The percentage RSD for memantine, donepezil was determined to be 0.9 and 0.3, respectively. For memantine and donepezil, recovery was obtained at 99.94% and 99.84%, respectively. Regression models for memantine and donepezil yielded LOD and LOQ values of 0.07 ppm, 0.20 ppm and 0.01 ppm, 0.04 ppm, respectively. The regression equations for memantine (y = 9960.2x + 51.857) and donepezil (y = 9514.8x + 793.79)