ANALYTICAL METHOD DEVELOPMENT VALIDATION FOR SIMULTANEOUS ESTIMATION OF PACLITAXEL, GEMCITABINE IN PH DOSAGE FORM BY HPLC

Abstract

Gemcitabine and Paclitaxel method validation was estimated by using Agilent column of dimension 150 x 4.6 mm, OPA: MeCN was selected as Mp in 55:45 v/v ratio. This combination was optimized at a spectrum of 256 nm. The Retention of this drugs were eluted at 2.205 and 3.080 for Gemcitabine and Paclitaxel, its RSD was 0.3 and 0.5 with in limit. And the regression was obtained at is y = 89619x + 2823.2, and y = 88164x + 5630.1 respectively. Mean recovery was achieved at 99.20% and 99.50% for Gemcitabine and Paclitaxel respectively and this method was specific with any interference of other drug peak.