A short review on DNA sequencing techniques

Authors

  • Sridhar Siddiraju Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Secunderabad, Telangana, India
  • Nishita Thota Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Secunderabad, Telangana, India

Keywords:

DNA sequencing, radioactive, human genome

Abstract

A DNA sequence is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequence is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine). DNA can be sequenced by a chemical procedure that breaks a terminally labelled DNA molecule partially at each repetition of a base. DNA sequencing is very significant in research and forensic science. The main objective of DNA sequence generation method is to evaluate the sequencing with very high accuracy and reliability. DNA sequencing can solve a lot of problems and perform a lot of work for human wellfare A sequencing can be done by different methods. Automated DNA sequencing is based on usage of flouroscent label and takes less time. Ion torrent semiconductor sequencing is based on the release of hydrogen ions and the pH of the solution lowers. Shot gun sequencing is used for larger fragments of DNA and it is widely used during human genome project. This article provides a comprehensive overview on DNA sequencing techniques in bioanalysis.

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Published

2020-06-01

How to Cite

Sridhar Siddiraju, & Nishita Thota. (2020). A short review on DNA sequencing techniques. World Journal of Pharmaceutical Sciences, 8(6), 127–130. Retrieved from https://wjpsonline.com/index.php/wjps/article/view/172

Issue

Section

Review Article