DETECTION AND ESTIMATION OF FLAVONOIDS PHENOLIC ACIDS AND XANTHONE IN HERBAL RAW MATERIALS BELONGS FAMILY OF CAESALPINACEAE, ACANTHACEAE, MENISPERMACEAE BY HPTLC TECHNIQUE.

Abstract

The prime aim of the study is to observe the flavonoids phenolic acid and xanthone in six commercial herbal raw materials namely Cassia lanceolata (Caesalpinaceae), Cassia auriculata flower (Caesalpinaceae), Cassia auriculata bark (Caesalpinaceae), Andrographis paniculata (Acanthaceae), Adhatoda vasica (Acanthaceae), Pachygone buxifolia (Menispermaceae). family respectively used in daily domestic needs to confirm the presence of common antioxidant secondary metabolites in herbal raw materials. Results of the study clearly revealed that this raw materials from the above family contains flavonoids and phenolic acids. The developed simultaneous HPTLC method can be employed for the routine investigations of flavonoids and phenolic acids in herbal raw materials and sample. The above-mentioned raw materials are procured from authenticated country shop.In Cassia lanceolata both quercetin & magniferin is absent. Cassia auriculata flower extract contains quercetin-0.49%, magniferin-0.04%. Cassia auriculata bark extract contains quercetin-1.38%, magniferin-0.06%. Andrographis paniculata extract contains quercetin-0.48%, magniferin-0.19%. Adhatoda vasica extract contain quercetin-0.57%, magniferin-0.08%. Pachygone buxifolia contain only quercetin-0.36%. In conclusion, both quercetin & magniferin present in Cassia auriculata flower, Cassia auriculata bark, Andrographis paniculata, Adhatoda vasica.In Cassia lanceolata both quercetin & magniferin is absent. Pachygone buxifolia contain only quercetin. Quercetin is present in maximum number of herbal extracts hence it possesses anti-oxidant property.