ESTIMATION OF ANTI-OXIDANT MARKERS IN TRIPHALA TABLET AND TRIPHALA CHOORNAM BY HPTLC TECHNIQUE

Abstract

The study aims to analyse flavonoids and phenolic acids in two indigenous herbal formulations: marketed formulation tablet, marketed formulation choornam, these formulations, commonly used in daily domestic needs, were examined to confirm the presence of antioxidant secondary metabolites in marketed formulations. Results revealed that both marketed formulation choornamontain flavonoids and phenolic acids. The developed simultaneous HPTLC method can be employed for routine investigations. The formulations were procured from a drug store and analysed for Quercetin, Rutin, Gallic acid, Tannic acid, Ellagic acid, Catechin, and Vitexin. In marketed formulation tablet, Quercetin (0.1617%), Rutin (0.41%), Gallic acid (1.60%), Tannic acid (0.51%), Ellagic acid (2.27%), Catechin (0.26%), and Vitexin (0.66%) were detected. In marketed formulation choornam, Quercetin (0.0008%), Rutin (0.85%), Gallic acid (1.0%), Tannic acid (1.06%), Ellagic acid (4.73%), and Catechin (0.56%) were found, but Vitexin was absent. In conclusion, key antioxidant markers—Tannic acid, Gallic acid, and Catechin—were present in both formulations. Marketed formulation tablet contained all tested compounds, whereas marketed formulation choornam lacked Vitexin. These findings confirm the presence of essential antioxidant constituents in both marketed formulations, reinforcing their therapeutic potential.