RP-HPLC Method Development and Validation for the Analysis of Levodopa and Carbidopa in Bulk and Tablet Dosage Forms

Abstract

the simultaneous evaluation of levodopa and carbidoxopa. The chromatogram was performed using Sunfire (250mm 4.6mm, 5μ). A mobile phase consisting of KH2PO4, acetonitrile in a 60:40 v/v ratio was injected across the column at a flow rate of 1.0 ml/min. The temperature was kept steady at 30°C. The optimal wavelength for levodopa and carbidogopa was 280 nm. Levodopa and carbidogpa's percentage RSDs were 1.5 and 0.6, respectively. The retention durations for levodopa and carbidogopa were found to be 2.183 and 2.755 minutes, respectively. The LOD and LOQ values obtained from the Levodopa and Carbidopa regression equations were 0.003, 0.009, and 0.06, 0.18, respectively. The regression equation for levodopa is y = 85498x + 14640, whereas that for carbidogopa is y = 80352x + 11509.