Genetic Analysis of MTB-H37RV by using VFDB-Database

Krishna Kumar Das; Sudeepa Rout; Yangya Prasad Nath Sharma; Lingaraj Jena

Authors

Krishna Kumar Das Department of Bioinformatics, C.P.G.S, OUAT, Bhubaneswar
Sudeepa Rout Department of Bioinformatics, C.P.G.S, OUAT, Bhubaneswar
Yangya Prasad Nath Sharma Department of Bioinformatics, C.P.G.S, OUAT, Bhubaneswar
Lingaraj Jena JBTDRC-MGIMS, Sevagram, Wardha, Maharashtra, India

Keywords:

MTB-H37Rv, Virulence factors, VFDB, STRING, GENE MANIA

Abstract

The virulence factor database (VFDB) is an integrated and comprehensive online resource for curating information about virulence factors of bacterial pathogens. Since its inception in 2004, VFDB has been dedicated to providing up-to-date knowledge of VFs from various medically significant bacterial pathogens. Virulence factors (VFs) refer to the properties (i.e., gene products) that enable a microorganism to establish itself on or within a host of a particular species and enhance its potential to cause disease. In my study by using this database we got 234 genes are associated with the Bacteria MTB-H37Rv. A total of 139 genes obtained from significantly enriched biological processes are termed as key genes that were carried out for network construction of MTB-H37Rv through STRING database. From STING we got the most interacting 68 genes in the bacteria which are called core genes for the disease TB and from GENE MANIA we get the common 13 genes associated with both the organism (MTB-H37Rv and Homo sapiens) during TB infection. The common genes of both the organisms are NFKB1, NFKB2, NFAT5, NFATC2, NFATC1, NFATC3, MMD, MMD2, ADIPOR2, PAQR3, PAQR5, PAQR6 and PAQR9.The structure and details of these genes are predicted by using bioinformatics tools and databases.