Method development and validation of paracetamol in bulk and tablet formulation by UV-Visible spectroscopy

Abstract

A  rapid,  simple,  selective  and  precise  UV-  Visible Spectrophotometric  method  has  been  developed for the determination of Paracetamol in bulk forms and solid dosage formulations. The spectrophotometric detection was carried out at an absorption maximum of 200 nm using methanol as solvent.  The method was validated for specificity, linearity, accuracy,    precision, and robustness. The detector response for the Paracetamol was linear over the selected concentration range 1 to 7 µg/ml with a correlation coefficient of 0.999.  The accuracy was between 99.92 & 100.94%.  The precision (R.S.D.) among six sample preparations was 0.30% (Intraday) & 0.59 % (Interday).  The LOD and LOQ are 0.480 and 1.457 µg/ml, respectively.  The recovery of Paracetamol was about 100.264%.  The results demonstrated that the excipients in the commercial tablets did not interfere with the method and can be conveniently employed for routine quality control analysis of Paracetamol in bulk drug, marketed tablets and other formulations.