Analytical Method Development and Validation of Abacavir in Pure and Pharmaceutical Dosage Forms by Using UV- Spectrophotometric Method

Abstract

A novel, simple, accurate and precise Zero order derivative spectroscopic method was developed and validated for the estimation of Abacavir in bulk and pharmaceutical dosage forms and has an absorption maximum at 296 nm in 0.1 N HCl. The Linearity was found to be in the concentration range of 2-16μg/ml and the correlation coefficient was found to be 0.999 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y = 0.045 X + 0.006. The % recovery values were found to be within 99.61-99.92 % showed that the method was accurate. The LOD and LOQ were found to be 0.1281 and 0.3843 mcg/ ml, respectively. The % RSD values were less than 2. The present methods were accomplishing the validation parameters according to ICH guidelines like accuracy, precision, linearity, range, ruggedness, limit of detection and limit of quantization etc. Proposed method was successfully applied for the quantitative estimation of Abacavir in bulk and pharmaceutical dosage forms.