Evaluation of Hypoglycemic Effect of Two flavonoidic Compounds Isolated from Iraqi Phoenix dactylifera (Gintar) Leaflets in Alloxan-Induced Diabetic Rabbits

Abstract

Diabetes Mellitus (DM) is a chronic metabolic disorder disease resulting from lack in action of insulin hormone. Thin Layer Chromatography (TLC) results showed presence of two flavonoidic spots from phoenix dactylifera (Gintar) leaflets have rate of flow equal to 0.35, 0.4. So, these compounds were separated by column chromatography (CC) then they were identified by infra-red spectrum and gas chromatography-mass spectrum technique and they were phenol,2,2^'-methylene bis[6-(1,1-dimethyl)-4-methyl and phenol,2,2^'-methylene bis [6-methoxy-3-(2-propenyl). Hypoglycemic effects of these active compounds were investigated and estimated in normal glycemic and hyperglycemic rabbits. The blood glucose levels in fasted normal rabbits were 137.66, 117.5, 106.88, 100.50 and 80.66 mg/100ml at 0,2,4,6 and 24 hr respectively from oral administration time. Also, the blood glucose levels in fasted diabetic rabbits were 375.83, 315.50, 213.66, 152.35 and 145.50 mg/100ml at 0,2,4,6 and 24 hr respectively. The two flavonoids had no toxic effect on hemolysis of red blood cells so these active compounds can be used safely to treat diabetes mellitus disease.