Ameliorative effect of curcumin and tannic acid on tumor-induced in female mice

Abstract

Curcumin, a natural polyphenol product of the plant Curcuma longa, has been shown to inhibit the growth and progression of cancer; Tannic acid, a naturally occurring dietary polyphenol, has been exerts chemopreventative activity against cancer in various animal models. This study was carried out on 220, 12-14 weeks old female mice and weighted 25-30gm. Mice were classified into two main large experiments. Experiment 1: Non-tumor bearing mice (NTB) Included 100 of animals and divided into four groups each one comprised 25 mice. Group 1: NTB-control saline treated. Group 2: NTB-treated with curcumin orally (350mg/kg/day) for 6 weeks. Group 3: NTB-treated with tannic acid orally (160mg/kg/day) for 6 weeks. Group 4: NTB-treated with curcumin and tannic acid orally at ratio (50%: 50%) for 6 weeks. Experiment 2: Tumor bearing (TB) mice. Included 120 of animals and divided into four groups each one comprised 30 mice. Group 1: TBM-control saline treated. Group 2: TBM-treated with curcumin orally (350mg/kg/day) for 6weeks. Group 3: TBM-treated with tannic acid orally (160mg/kg/day) for 6weeks. Group 4: TBM-treated with curcumin and tannic acid orally at ratio (50%: 50%) for 6 weeks. Blood samples were collected from all animals groups after 2, 4 and 6 weeks from treatment. Serum were separated and processed directly for (AST and ALT) activities, (urea, creatinine, L-MDA) concentration and catalase activity determination. The obtained results revealed that, a very highly significant increase in serum AST, ALT, urea, creatinine and L-MDA concentration. On contrary, a highly significant decrease in serum catalase activity was observed in tumor bearing mice when compared with control. The results of this study indicated that curcumin, tannic acid and their combination treatment have potential benefits in cancer treatment.