Chemical and Cytotoxic activities Study of Cuscuta campestris Yunck

Mai Salah Hendawy; Zain M. Naeem; Mohamed Farid Lahloub; Mohamed M.A. Amer

Authors

Mai Salah Hendawy Department of Pharmacognosy, Faculty of Pharmacy, Horus University, New Damietta, Egypt
Zain M. Naeem Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt
Mohamed Farid Lahloub Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt
Mohamed M.A. Amer Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura-35516, Egypt

Keywords:

Cuscuta campestris, cytotoxic activities, SRB assay, phytochemical investigation

Abstract

The purpose of this study was to investigate chemical constituents and biological activity of different extracts on different human cell lines. The phytochemical investigation of whole parasitic Cuscuta campestris has led to the isolation and identification of β-sitosterol (1), stigmasterol (2), (1s,2s)-1,2,3-trihydroxy-1(3',4'-methylenedioxyphenyl) propane (3) isolated for first time from Cuscuta campestris, d-sesamin (4), oleanolic acid (5), linocinnamarin (6) isolated for first time from Cuscuta campestris, mixture of isomers of quercetin-3-O-β-D-glucoside and quercetin-3-O-β-D-galactoside (7,8), astragallin (9). The methylene chloride, petroleum ether and ethyl acetate fractions were tested against human lung cancer (A-549), human colorectal (cecum) cancer (LS-513), human cervical cancer (Hela), human ovarian cancer (SKOV-3), human bladder cancer (T-24) and human pancreatic cancer (PANC-1) by using SRB assay. The ethyl acetate and petroleum ether fractions showed moderate to week cytotoxic activities, but methylene chloride fraction showed strong cytotoxic activity on all tested cell lines.