Antibacterial activity of Eucalyptus oblique aqueous leaf extract on selective Vibrio species of Penaeus monodon culture hatchery

Abstract

The anti-bacterial activity of aqueous leaf extract of Eucalyptus oblique against selective Vibrio spp was evaluated. Vibrios are marine bacterium, responsible for disease outbreak affecting commercial production of shrimp species especially Penaeus monodon.  Vibrios were cultivated on Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and seven colonies of vibrio spp. were isolated based on their colony morphology.  The aqueous plant extract was prepared from Eucalyptus oblique and its antibacterial activity evaluated against selected Vibrio spp. The antibacterial activity was tested by using the disk diffusion method, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods.