Antibacterial potential of selective aqueous plant extracts against Vibrio parahaemolyticus

Abstract

To evaluate the anti-bacterial activity of the selected plants, Lawsonia inermis (Lythraceae), Phyllathus niruri (Phyllanthaceae) and Eucalyptus kitsoniana (Myrtaceae) against V.parahaemolyticus.   Which is a marine bacterium, responsible for disease outbreak affecting commercial production of shrimp species especially in Penaeus monodon.    In this study, twenty seven colonies of V. parahaemolyticus, were isolated by cultivating in TCBS agar.    The colonies were analyzed by bio chemical assay and PCR method confirming that it was V. parahaemolyticus.   The aqueous plant extracts were prepared from the selected plant samples and evaluated for their antibacterial activity against V. parahaemolyticus.  The antibacterial activity was tested by using the disk diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods.