Bactericidal effect of Lawsonia inermis aqueous leaf extract against selective Vibrio species of Penaeus monodon culture hatchery

Abstract

The bactericidal effect of aqueous leaf extract of Lawsonia inermis against selective Vibrio species was evaluated. Vibrios are marine bacterium, responsible for disease outbreak affecting commercial production of shrimp species especially Penaeus monodon.  Vibrios were cultivated on Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and seven colonies of Vibrio species were isolated based on their colony morphology.  The aqueous plant extract was prepared from Lawsonia inermis and its antibacterial activity evaluated against selected Vibrio species. The antibacterial activity was tested by using the disk diffusion method, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods.