Quantitative simultaneous determination of montlukast and bambuterol in combined tablet formulation by RP-HPLC method
Keywords:Bambuterol, Montelukast, RP-HPLC, Method Development, Validation
A simple, Accurate, precise method was developed for the simultaneous estimation of the Bambuterol and Montelukast in bulk and Tablet dosage form. Chromatogram was run through Std Discovery 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1.0 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 254.0 nm. Retention time of Bambuterol and Montelukast were found to be 2.067min and 3.032 min. %RSD of the Bambuterol and Montelukast were and found to be 0.6% and 0.4% respectively. %Recovery was obtained as 100.12% and 99.96% for Bambuterol and Montelukast respectively. LOD, LOQ values obtained from regression equations of Bambuterol and Montelukast were 0.09, 0.26 and 0.14, 0.42respectively. %Assay was obtained as 99.97% and 100.02% for Bambuterol and Montelukast respectively. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
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