Bactericidal activity of Ocimum tenuiflorum leaf extract against selective Vibrio species of Penaeus monodon culture hatchery

Abstract

The bactericidal activity of aqueous leaf extract of Ocimum tenuiflorum against selective Vibrio species was evaluated. Vibrios are marine bacterium, responsible for disease outbreak affecting commercial production of shrimp species especially Penaeus monodon.  Vibrios were cultivated on Thiosulfate-citrate-bile salts-sucrose (TCBS) agar and seven colonies of Vibrio species were isolated based on their colony morphology.  The plant extract was prepared from Ocimum tenuiflorum and its antibacterial activity was evaluated against Vibrio species. The antibacterial activity was tested by using the disk diffusion method, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods.