Simultaneous Estimation of Montelukast and Bilastine in Bulk and Pharmaceutical Dosage Form by RP-HPLC

Abstract

A simple, accurate, precise method was developed to estimate Bilastine and Montelukast in bulk and tablet form. Chromatogram was run on Std Inertsil C18 150 x 4.6 mm, 5m. Mobile phase with Buffer 0.01N Na2HPO4: Acetonitrile 70:30 was pumped through column at 1.0ml/min. Method buffer was 0.01N Na2hpo4. The temperature was 30°C. The optimal wavelength was 218nm. Bilastine and Montelukast had 2.524 and 3.153min retention times. Bilastine and Montelukast had 0.9 and 1.2 %RSD. Recovery was 99.98% for Bilastine and 99.48% for Montelukast. Bilastine and Montelukast regression equations yielded LOD, LOQ values of 0.32, 0.97, and 0.24, 0.72. Montelukast regression equation is y = 36464x + 8987, while Bilastine is y = 31133x + 12857. The method developed was simple and economical for regular quality control tests in industries because retention and run times were reduced.