Wound healing potential of tannin on dead space wound in diabetic rats

Abstract

Butea or Palas gum is a tree bark exudate that comes from the stems naturally or as a result of the plant being wounded. Tannin was isolated from palas gum by dissolving it in boiling water and then treating the cool filtrate with a saturated brine solution to create a precipitate. The precipitated Tannin was extracted with alcohol, yielding 42 percent, which was confirmed by calculating the Tannin as Tannic Acid Equivalent. Tannin's effect on the healing of rat dermal wounds was studied in diabetic rats utilising an in vivo dead space wound model. On each axilla of diabetic rats, dead space incisions were created. For eight days, the rats were randomly assigned to one of three treatment groups (Group I: Normal saline; Group II: Diabetic control; Group III: Tannin). Animals were euthanized on day 10, and cotton pellets and granuloma tissues were carefully collected and processed for further estimates. When compared to the control, the levels of hydroxyl proline, hexuronic acid, tissue protein, and lysyl oxidase were considerably higher. These findings substantiate Tannin's positive benefits in the speeding up of the healing process. As a result, the current study backs up the plant's wound healing claims in diabetic wounds.